Journal:
Article Title: Pneumococcal Conjugate Vaccines Overcome Splenic Dependency of Antibody Response to Pneumococcal Polysaccharides
doi: 10.1128/IAI.69.12.7583-7587.2001
Figure Lengend Snippet: IgG responses to vaccination with PPSs (left panels) and PPS-protein conjugates (right panels) in control and splenectomized rats. Control rats (filled symbols) and splenectomized rats (open symbols) were vaccinated i.v. and s.c. at days 0 and 28 either with 2.5 μg of each of PPS serotypes 4, 6B, 9V, 14, 19F, and 23F or with a tetravalent, PCV consisting of polysaccharides of pneumococcal serotypes 6B, 14, 19F, and 23F conjugated to tetanus toxoid. Left panels show IgG responses at days 0, 5, 28, 33, and 56 after vaccination with PPS. Antibody responses to serotypes 4 and 9V are not shown but displayed a course similar to that for serotype 6B. Right panels show IgG responses at days 0, 5, 28, 33, and 56 after vaccination with PCV. Antibody titers are expressed in units per milliliter. Shown are geometric mean antibody titers and standard errors of the means.
Article Snippet: Microtiter plates (Greiner Labortechnik, Langerthal, Germany) were coated with pneumococcal capsular polysaccharides (ATCC; 10 μg/ml in 0.9% NaCl) and stored overnight at 37°C.
Techniques: Control